Cryo-EM structure of 90S small ribosomal subunit precursors in …
Sep 18, 2020 · The formation of eukaryotic ribosomes is a complex process that starts with transcription of a large precursor RNA that assembles into a large 90S preribosome, which matures to finally give the 40S small subunit of the ribosome.
mRNA reading frame maintenance during eukaryotic ribosome
Nov 29, 2023 · We demonstrate how the accuracy of eukaryotic translocation relies on eukaryote-specific elements of the 80S ribosome, eEF2 and tRNAs. Our findings shed light on the mechanism of...
Nucleolar maturation of the human small subunit processome | Science …
We report the high-resolution cryo–electron microscopy structures of maturing human small subunit (SSU) processomes at resolutions of 2.7 to 3.9 angstroms. These structures reveal the molecular mechanisms that enable crucial progressions during SSU processome maturation.
Principles of mitoribosomal small subunit assembly in eukaryotes
Dec 8, 2022 · By revealing the dynamic interplay between assembly factors, mitoribosomal proteins and rRNA that are required to generate functional subunits, our structural analysis provides a vignette for how...
Ribosome biogenesis in disease: new players and therapeutic …
Jan 9, 2023 · The eukaryotic ribosome includes two subunits, the 40S small subunit, and the 60S large subunit, which combine to form the 80S ribosome with translational activity. 8 The 40S subunit is ...
Single-molecule tracking reveals dynamic regulation of ribosomal …
Oct 2, 2024 · During eukaryotic translation initiation, a 43S pre-initiation complex (PIC) of the small (40S) ribosomal subunit, the methionylated initiator tRNA (Met-tRNA iMet) and protein initiation factors, loads at the mRNA 5′ end (1).
ZNF692 organizes a hub specialized in 40S ribosomal subunit …
Here, we report the identification of the zinc finger protein 692 (ZNF692), a MYC-induced nucleolar scaffold that coordinates the final steps in the biogenesis of the small ribosome subunit.
Yeast Eukaryotic Initiation Factor 4B Remodels the MRNA Entry …
Eukaryotic Initiation Factor 4 (eIF4) is a group of factors that activates mRNA for translation and recruit 43S preinitiation complex (PIC) to the mRNA 5′ end, forming the 48S PIC. The eIF4 factors include mRNA 5′ cap-binding protein eIF4E, ATP-dependent RNA helicase eIF4A, and scaffold protein eIF4G, which anchors eIF4A and eIF4E. Another …
An emerging mechanism for the maturation of the Small Subunit ...
The biogenesis of the eukaryotic ribosome is a tightly regulated and energetically demanding process involving more than 200 ribosome assembly factors. These factors work in concert to ensure accurate assembly and maturation of both ribosomal subunits.
Probing small ribosomal subunit RNA helix 45 acetylation across ...
Jun 2, 2022 · NAT10 is an essential enzyme that catalyzes N4-acetylcytidine (ac4C) in eukaryotic transfer RNA and 18S ribosomal RNA. Recent studies suggested that rRNA acetylation is dependent on SNORD13, a box C/D small nucleolar RNA predicted to base-pair with 18S rRNA via two antisense elements.