Somatic hypermutation (SHM) of immunoglobulin variable (V) regions modulates antibody-antigen affinity is initiated by activation-induced cytidine deaminase (AID) on single-stranded DNA (ssDNA).

We have used an in vitro chromatin assembly and transcription system to examine the biochemistry of interactions among ERα, the SRC proteins and p300/CBP. Using polypeptides designed to block specific ...