In this article, we’ll briefly discuss several RNA-seq workflows, how they differ, and the advantages and drawbacks of each. Regardless of the method you choose, every RNA-seq project begins with ...

RNA sequencing (RNA-seq) is a powerful tool for molecular biologists ... 2 Scientists are designing FFPE sample-tailored RNA library preparation protocols, reagents, and kits to remove this bottleneck ...

A new statistical technique developed by a researcher at the Texas A&M University School of Public Health and colleagues ...

Here, we look at why RNA-seq is useful, how the technique works, a basic protocol that is commonly used today and challenges that remain.

RNA-sequencing has become a cornerstone in the study of gene expression, offering insights beyond mere mRNA transcript ...

By exploring single cell RNA-sequencing data from clinical trials using CRISPR-Cas9 engineered T cells, the researchers found that a clinical trial using a different protocol, where scientists edited ...

Owing to the protocol, which is based on oligo-dT priming followed by A-tailing of cDNA and subsequent cDNA fragmentation before sequencing, the direction of the RNA is lost. There are no ...

RNA modulates transcription factor binding to DNA, though the subsequent effect on transcription remains unclear. The study ...

MDA5 is a critical cytoplasmic RNA sensor capable of triggering type I interferon pathways. This study shows that the E3 ...

Small RNA molecules known as short noncoding RNAs show up in the body in response to an infection. Sequencing RNA from a blood sample is one way to find out what kind and how much of different RNA ...

For the study, the team developed a protocol for enriching brain endothelial cells in ... "The results complement and expand findings from studies on the RNA sequencing of brain endothelial cells ...