One involves studying the effect of a 'candidate' gene on a phenotype; for example, using transgenesis or homologous recombination (creating 'knockout' or 'knockin' mice). The other entails using ...

Homologous recombination plays essential roles in the repair of DNA double strand breaks (DSBs) and collapsed replication forks. The fundamental reaction in homologous recombination is the exchange of ...

It is thought that these proteins play a role in preventing recombination during DNA replication when it is not needed. An alternate hypothesis proposes that homologous chromosomes reside in pairs ...

Both gene knockout and knock-in models are powerful ... or nonhomologous end-joining (NHEJ). Historically, homologous ...

To illuminate these issues, our lab has developed and refined methods for precisely mutating genes in human somatic cells via homologous recombination ... (i.e., conditional or constitutive “knockout” ...