With this in mind, it is possible to calculate the absorbance of the sample with the following equation ... employed in the calculation of analyte concentration. The software will automatically ...

The absorbance is directly proportional to the sample's concentration, enabling accurate quantification. Fluorophores bind to the sample of interest and are excited by a specific wavelength of ...

If it does not, increase the concentration of horseradish peroxidase ... or when the initial linear or pseudo-linear increase in absorbance with time is lost, and the curve tends towards a ...